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While powerful tools for non-invasive, longitudinal, in vivo assessment, MRI, DTI, and MTR do not allow for microscopic assessment and can be confounded by edema, although this issue is not a particular consideration for the cuprizone model given that vasogenic edema does not play a prominent role
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325,326 It is interesting to note that although this three-coordinate Pb II -pyramidyl structure may well be a common feature of Pb II -responsive regulators, the presence of four Cys residues in the metal binding site of CadC negatively impacts Pb II specificity over Cd II or Zn II